LOCUS       pLV-[hsa-miR-1262]        9147 bp    DNA     circular     27-JUN-2007
SOURCE      
COMMENT     
COMMENT     This file is created by Biosettia Inc.
            http://www.biosettia.com/
FEATURES             Location/Qualifiers
     misc_feature    1807..1929
                     /label=cPPT
     CDS             7068..7928
                     /label=Amp(R)
     promoter        8129..8669
                     /label=pUC ori
     promoter        7..235
                     /label=RSV
     LTR             5673..5907
                     /label=3' LTR
     intron          526..1690
                     /label=Intron
     rep_origin      complement(6482..6937)
                     /label=f1 ori
     LTR             236..416
                     /label=5'LTR
     polyA_signal    5979..6109
                     /label=SV40 pA
     misc_feature    6110..6323
                     /label=SV40 ori
     misc_feature    4994..5590
                     /label=WPRE
     promoter        1933..2328
                     /label=human EF1a promoter
     intron          2361..3612
                     /label=intron
     CDS             3659..4984
                     /label=rPuro (RFP Puro)
     misc_RNA        3110..3402
                     /label=hsa-mir-1262 precursor
BASE COUNT     2195 a      2354 c      2466 g      2132 t 
ORIGIN
        1 aagcttaatg tagtcttatg caatactctt gtagtcttgc aacatggtaa cgatgagtta 
       61 gcaacatgcc ttacaaggag agaaaaagca ccgtgcatgc cgattggtgg aagtaaggtg 
      121 gtacgatcgt gccttattag gaaggcaaca gacgggtctg acatggattg gacgaaccac 
      181 tgaattgccg cattgcagag atattgtatt taagtgccta gctcgataca taaacgggtc 
      241 tctctggtta gaccagatct gagcctggga gctctctggc taactaggga acccactgct 
      301 taagcctcaa taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga 
      361 ctctggtaac tagagatccc tcagaccctt ttagtcagtg tggaaaatct ctagcagtgg 
      421 cgcccgaaca gggacttgaa agcgaaaggg aaaccagagg agctctctcg acgcaggact 
      481 cggcttgctg aagcgcgcac ggcaagaggc gaggggcggc gactggtgag tacgccaaaa 
      541 attttgacta gcggaggcta gaaggagaga gatgggtgcg agagcgtcag tattaagcgg 
      601 gggagaatta gatcgcgatg ggaaaaaatt cggttaaggc cagggggaaa gaaaaaatat 
      661 aaattaaaac atatagtatg ggcaagcagg gagctagaac gattcgcagt taatcctggc 
      721 ctgttagaaa catcagaagg ctgtagacaa atactgggac agctacaacc atcccttcag 
      781 acaggatcag aagaacttag atcattatat aatacagtag caaccctcta ttgtgtgcat 
      841 caaaggatag agataaaaga caccaaggaa gctttagaca agatagagga agagcaaaac 
      901 aaaagtaaga ccaccgcaca gcaagcggcc gctgatcttc agacctggag gaggagatat 
      961 gagggacaat tggagaagtg aattatataa atataaagta gtaaaaattg aaccattagg 
     1021 agtagcaccc accaaggcaa agagaagagt ggtgcagaga gaaaaaagag cagtgggaat 
     1081 aggagctttg ttccttgggt tcttgggagc agcaggaagc actatgggcg cagcgtcaat 
     1141 gacgctgacg gtacaggcca gacaattatt gtctggtata gtgcagcagc agaacaattt 
     1201 gctgagggct attgaggcgc aacagcatct gttgcaactc acagtctggg gcatcaagca 
     1261 gctccaggca agaatcctgg ctgtggaaag atacctaaag gatcaacagc tcctggggat 
     1321 ttggggttgc tctggaaaac tcatttgcac cactgctgtg ccttggaatg ctagttggag 
     1381 taataaatct ctggaacaga tttggaatca cacgacctgg atggagtggg acagagaaat 
     1441 taacaattac acaagcttaa tacactcctt aattgaagaa tcgcaaaacc agcaagaaaa 
     1501 gaatgaacaa gaattattgg aattagataa atgggcaagt ttgtggaatt ggtttaacat 
     1561 aacaaattgg ctgtggtata taaaattatt cataatgata gtaggaggct tggtaggttt 
     1621 aagaatagtt tttgctgtac tttctatagt gaatagagtt aggcagggat attcaccatt 
     1681 atcgtttcag acccacctcc caaccccgag gggacccgac aggcccgaag gaatagaaga 
     1741 agaaggtgga gagagagaca gagacagatc cattcgatta gtgaacggat ctcgacggta 
     1801 tcggttaact tttaaaagaa aaggggggat tggggggtac agtgcagggg aaagaatagt 
     1861 agacataata gcaacagaca tacaaactaa agaattacaa aaacaaatta caaaattcaa 
     1921 aattttatcg atgcctcccc gtcaccaccc cccccaaccc gccccgaccg gagctgagag 
     1981 taattcatac aaaaggactc gcccctgcct tggggaatcc cagggaccgt cgttaaactc 
     2041 ccactaacgt agaacccaga gatcgctgcg ttcccgcccc ctcacccgcc cgctctcgtc 
     2101 atcactgagg tggagaagag catgcgtgag gctccggtgc ccgtcagtgg gcagagcgca 
     2161 catcgcccac agtccccgag aagttggggg gaggggtcgg caattgaacc ggtgcctaga 
     2221 gaaggtggcg cggggtaaac tgggaaagtg atgtcgtgta ctggctccgc ctttttcccg 
     2281 agggtggggg agaaccgtat ataagtgcag tagtcgccgt gaacgttctt tttcgcaacg 
     2341 ggtttgccgc cagaacacag gtaagtgccg tgtgtggttc ccgcgggcct ggcctcttta 
     2401 cgggttatgg cccttgcgtg ccttgaatta cttccacgcc cctggctgca gtacgtgatt 
     2461 cttgatcccg agcttcgggt tggaagtggg tgggagagtt cgaggccttg cgcttaagga 
     2521 gccccttcgc ctcgtgcttg agttgaggcc tggcctgggc gctggggccg ccgcgtgcga 
     2581 atctggtggc accttcgcgc ctgtctcgct gctttcgata agtctctagc catttaaaat 
     2641 ttttgatgat atcctgcgac gctttttttc tggcaagata gtcttgtaaa tgcgggccaa 
     2701 gatctgcaca ctggtatttc ggtttttggg gccgcgggcg gcgacggggc ccgtgcgtcc 
     2761 cagcgcacat gttcggcgag gcggggcctg cgagcgcggc caccgagaat cggacggggg 
     2821 tagtctcaag ctggccggcc tgctctggtg cctggcctcg cgccgccgtg tatcgccccg 
     2881 ccctgggcgg caaggctggc ccggtcggca ccagttgcgt gagcggaaag atggccgctt 
     2941 cccggccctg ctgcagggag ctcaaaatgg aggacgcggc gctcgggaga gcgggcgggt 
     3001 gagtcaccca cacaaaggaa aagggccttt ccgtcctcag ccgtcgcttc atgtgactcc 
     3061 acggagtacc gggcgccgtc caggcacctc gattagttct cgaggatcca agtggacatc 
     3121 aagggaagac aactggaata aagggaacaa tctattgatc ataaacaatt ctctgaattt 
     3181 catccttcta caaattcacc cctcacatta tctacaatgg tgatgggtga atttgtagaa 
     3241 ggatgaaagt caaagaatcc ttctgggaac taatttttgg ccttcaacaa gaattgtgat 
     3301 atttaattct gtgttttctg atgcttctga ggaaagctcc ataaagagag ctgaatgggg 
     3361 cagaggattt tatacactat aagaactatc cctgctatga tggctagctc gagcttttgg 
     3421 agtacgtcgt ctttaggttg gggggagggg ttttatgcga tggagtttcc ccacactgag 
     3481 tgggtggaga ctgaagttag gccagcttgg cacttgatgt aattctcctt ggaatttgcc 
     3541 ctttttgagt ttggatcttg gttcattctc aagcctcaga cagtggttca aagttttttt 
     3601 cttccatttc aggtgtcgtg aaaactaccc ctctagagtc gagctaccgg tcgccaccat 
     3661 ggtgagcaag ggcgaggagg ataacatggc catcatcaag gagttcatgc gcttcaaggt 
     3721 gcacatggag ggctccgtga acggccacga gttcgagatc gagggcgagg gcgagggccg 
     3781 cccctacgag ggcacccaga ccgccaagct gaaggtgacc aagggtggcc ccctgccctt 
     3841 cgcctgggac atcctgtccc ctcagttcat gtacggctcc aaggcctacg tgaagcaccc 
     3901 cgccgacatc cccgactact tgaagctgtc cttccccgag ggcttcaagt gggagcgcgt 
     3961 gatgaacttc gaggacggcg gcgtggtgac cgtgacccag gactcctccc tgcaggacgg 
     4021 cgagttcatc tacaaggtga agctgcgcgg caccaacttc ccctccgacg gccccgtaat 
     4081 gcagaagaag accatgggct gggaggcctc ctccgagcgg atgtaccccg aggacggcgc 
     4141 cctgaagggc gagatcaagc agaggctgaa gctgaaggac ggcggccact acgacgctga 
     4201 ggtcaagacc acctacaagg ccaagaagcc cgtgcagctg cccggcgcct acaacgtcaa 
     4261 catcaagttg gacatcacct cccacaacga ggactacacc atcgtggaac agtacgaacg 
     4321 cgccgagggc cgccactcca ccggcggcat ggacgagctg tacaaggacc caccggtcgc 
     4381 caccatgacc gagtacaagc ccacggtgcg cctcgccacc cgcgacgacg tccccagggc 
     4441 cgtacgcacc ctcgccgccg cgttcgccga ctaccccgcc acgcgccaca ccgtcgatcc 
     4501 ggaccgccac atcgagcggg tcaccgagct gcaagaactc ttcctcacgc gcgtcgggct 
     4561 cgacatcggc aaggtgtggg tcgcggacga cggcgccgcg gtggcggtct ggaccacgcc 
     4621 ggagagcgtc gaagcggggg cggtgttcgc cgagatcggc ccgcgcatgg ccgagttgag 
     4681 cggttcccgg ctggccgcgc agcaacagat ggaaggcctc ctggcgccgc accggcccaa 
     4741 ggagcccgcg tggttcctgg ccaccgtcgg cgtctcgccc gaccaccagg gcaagggtct 
     4801 gggcagcgcc gtcgtgctcc ccggagtgga ggcggccgag cgcgccgggg tgcccgcctt 
     4861 cctggagacc tccgcgcccc gcaacctccc cttctacgag cggctcggct tcaccgtcac 
     4921 cgccgacgtc gagtgcccga aggaccgcgc gacctggtgc atgacccgca agcccggtgc 
     4981 ctgagcggcc gcgtcgacaa tcaacctctg gattacaaaa tttgtgaaag attgactggt 
     5041 attcttaact atgttgctcc ttttacgcta tgtggatacg ctgctttaat gcctttgtat 
     5101 catgctattg cttcccgtat ggctttcatt ttctcctcct tgtataaatc ctggttgctg 
     5161 tctctttatg aggagttgtg gcccgttgtc aggcaacgtg gcgtggtgtg cactgtgttt 
     5221 gctgacgcaa cccccactgg ttggggcatt gccaccacct gtcagctcct ttccgggact 
     5281 ttcgctttcc ccctccctat tgccacggcg gaactcatcg ccgcctgcct tgcccgctgc 
     5341 tggacagggg ctcggctgtt gggcactgac aattccgtgg tgttgtcggg gaagctgacg 
     5401 tcctttccat ggctgctcgc ctgtgttgcc acctggattc tgcgcgggac gtccttctgc 
     5461 tacgtccctt cggccctcaa tccagcggac cttccttccc gcggcctgct gccggctctg 
     5521 cggcctcttc cgcgtcttcg ccttcgccct cagacgagtc ggatctccct ttgggccgcc 
     5581 tccccgcctg gaattcgagc tcggtacctt taagaccaat gacttacaag gcagctgtag 
     5641 atcttagcca ctttttaaaa gaaaaggggg gactggaagg gctaattcac tcccaacgaa 
     5701 gacaagatct gctttttgct tgtactgggt ctctctggtt agaccagatc tgagcctggg 
     5761 agctctctgg ctaactaggg aacccactgc ttaagcctca ataaagcttg ccttgagtgc 
     5821 ttcaagtagt gtgtgcccgt ctgttgtgtg actctggtaa ctagagatcc ctcagaccct 
     5881 tttagtcagt gtggaaaatc tctagcagta gtagttcatg tcatcttatt attcagtatt 
     5941 tataacttgc aaagaaatga atatcagaga gtgagaggaa cttgtttatt gcagcttata 
     6001 atggttacaa ataaagcaat agcatcacaa atttcacaaa taaagcattt ttttcactgc 
     6061 attctagttg tggtttgtcc aaactcatca atgtatctta tcatgtctgg ctctagctat 
     6121 cccgccccta actccgccca tcccgcccct aactccgccc agttccgccc attctccgcc 
     6181 ccatggctga ctaatttttt ttatttatgc agaggccgag gccgcctcgg cctctgagct 
     6241 attccagaag tagtgaggag gcttttttgg aggcctaggg acgtacccaa ttcgccctat 
     6301 agtgagtcgt attacgcgcg ctcactggcc gtcgttttac aacgtcgtga ctgggaaaac 
     6361 cctggcgtta cccaacttaa tcgccttgca gcacatcccc ctttcgccag ctggcgtaat 
     6421 agcgaagagg cccgcaccga tcgcccttcc caacagttgc gcagcctgaa tggcgaatgg 
     6481 gacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc 
     6541 gctacacttg ccagcgccct agcgcccgct cctttcgctt tcttcccttc ctttctcgcc 
     6601 acgttcgccg gctttccccg tcaagctcta aatcgggggc tccctttagg gttccgattt 
     6661 agtgctttac ggcacctcga ccccaaaaaa cttgattagg gtgatggttc acgtagtggg 
     6721 ccatcgccct gatagacggt ttttcgccct ttgacgttgg agtccacgtt ctttaatagt 
     6781 ggactcttgt tccaaactgg aacaacactc aaccctatct cggtctattc ttttgattta 
     6841 taagggattt tgccgatttc ggcctattgg ttaaaaaatg agctgattta acaaaaattt 
     6901 aacgcgaatt ttaacaaaat attaacgctt acaatttagg tggcactttt cggggaaatg 
     6961 tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga 
     7021 gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg agtattcaac 
     7081 atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt tttgctcacc 
     7141 cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga gtgggttaca 
     7201 tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc 
     7261 caatgatgag cacttttaaa gttctgctat gtggcgcggt attatcccgt attgacgccg 
     7321 ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt gagtactcac 
     7381 cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc agtgctgcca 
     7441 taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga ggaccgaagg 
     7501 agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat cgttgggaac 
     7561 cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct gtagcaatgg 
     7621 caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc cggcaacaat 
     7681 taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg gcccttccgg 
     7741 ctggctggtt tattgctgat aaatctggag ccggtgagcg tgggtctcgc ggtatcattg 
     7801 cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg acggggagtc 
     7861 aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca ctgattaagc 
     7921 attggtaact gtcagaccaa gtttactcat atatacttta gattgattta aaacttcatt 
     7981 tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt 
     8041 aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt 
     8101 gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag 
     8161 cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta actggcttca 
     8221 gcagagcgca gataccaaat actgttcttc tagtgtagcc gtagttaggc caccacttca 
     8281 agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg 
     8341 ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg 
     8401 cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct 
     8461 acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga 
     8521 gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc 
     8581 ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg 
     8641 agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg 
     8701 cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt 
     8761 tatcccctga ttctgtggat aaccgtatta ccgcctttga gtgagctgat accgctcgcc 
     8821 gcagccgaac gaccgagcgc agcgagtcag tgagcgagga agcggaagag cgcccaatac 
     8881 gcaaaccgcc tctccccgcg cgttggccga ttcattaatg cagctggcac gacaggtttc 
     8941 ccgactggaa agcgggcagt gagcgcaacg caattaatgt gagttagctc actcattagg 
     9001 caccccaggc tttacacttt atgcttccgg ctcgtatgtt gtgtggaatt gtgagcggat 
     9061 aacaatttca cacaggaaac agctatgacc atgattacgc caagcgcgca attaaccctc 
     9121 actaaaggga acaaaagctg gagctgc 
//
